Single-cell transcriptome of bronchoalveolar lavage fluid reveals sequential change of macrophages during SARS-CoV-2 infection in ferrets
DC Field | Value | Language |
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dc.contributor.author | Lee, Jeong Seok | - |
dc.contributor.author | Koh, June-Young | - |
dc.contributor.author | Yi, Kijong | - |
dc.contributor.author | Kim, Young-Il | - |
dc.contributor.author | Park, Su-Jin | - |
dc.contributor.author | Kim, Eun-Ha | - |
dc.contributor.author | Kim, Se-Mi | - |
dc.contributor.author | Park, Sung Ho | - |
dc.contributor.author | Ju, Young Seok | - |
dc.contributor.author | Choi, Young Ki | - |
dc.contributor.author | Park, Su-Hyung | - |
dc.date.accessioned | 2022-12-26T10:15:33Z | - |
dc.date.available | 2022-12-26T10:15:33Z | - |
dc.date.issued | 2021-07-27 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.issn | 2041-1723 | - |
dc.identifier.uri | https://scholarworks.gnu.ac.kr/handle/sw.gnu/3473 | - |
dc.description.abstract | Few studies have used a longitudinal approach to describe the immune response to SARS-CoV-2 infection. Here, we perform single-cell RNA sequencing of bronchoalveolar lavage fluid cells longitudinally obtained from SARS-CoV-2-infected ferrets. Landscape analysis of the lung immune microenvironment shows distinct changes in cell proportions and characteristics compared to uninfected control, at 2 and 5 days post-infection (dpi). Macrophages are classified into 10 distinct subpopulations with transcriptome changes among monocyte-derived infiltrating macrophages and differentiated M1/M2 macrophages, notably at 2 dpi. Moreover, trajectory analysis reveals gene expression changes from monocyte-derived infiltrating macrophages toward M1 or M2 macrophages and identifies a macrophage subpopulation that has rapidly undergone SARS-CoV-2-mediated activation of inflammatory responses. Finally, we find that M1 or M2 macrophages show distinct patterns of gene modules downregulated by immune-modulatory drugs. Overall, these results elucidate fundamental aspects of the immune response dynamics provoked by SARS-CoV-2 infection. A longitudinal analysis of SARS-CoV-2 infection in humans is challenging. Here the authors show a single cell RNA-sequencing analysis of BAL fluid cells from ferrets and characterise the time dependent recruitment of macrophage subsets to the lungs in response to SARS-CoV-2 infection. | - |
dc.language | 영어 | - |
dc.language.iso | ENG | - |
dc.publisher | NATURE PORTFOLIO | - |
dc.title | Single-cell transcriptome of bronchoalveolar lavage fluid reveals sequential change of macrophages during SARS-CoV-2 infection in ferrets | - |
dc.type | Article | - |
dc.publisher.location | 독일 | - |
dc.identifier.doi | 10.1038/s41467-021-24807-0 | - |
dc.identifier.scopusid | 2-s2.0-85111612748 | - |
dc.identifier.wosid | 000683367300030 | - |
dc.identifier.bibliographicCitation | NATURE COMMUNICATIONS, v.12, no.1 | - |
dc.citation.title | NATURE COMMUNICATIONS | - |
dc.citation.volume | 12 | - |
dc.citation.number | 1 | - |
dc.type.docType | Article | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.subject.keywordPlus | SET ENRICHMENT ANALYSIS | - |
dc.subject.keywordPlus | INFLUENZA-VIRUSES | - |
dc.subject.keywordPlus | PPAR-GAMMA | - |
dc.subject.keywordPlus | TRANSMISSION | - |
dc.subject.keywordPlus | MONOCYTES | - |
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