사슴의 개체 식별 및 친자 확인을 위한 microsatellite 마커 세트 구축

Abstract

Deer is mainly raised for the deer velvet antler used as herbal medicine in Korea, but the self-sufficiency rate of deer velvet antlers in Korea is low and there is no systematic system. Therefore, this study was conducted to establish a microsatellite (MS) marker set for individual identification and paternity test of elk (Cervus canadensis), red deer (Cervus elaphus), and sika deer (Cervus nippon), which are mainly raised in Korea. A total of 9 MS markers were selected and combined into one set based on the size of the marker, the heterozygosity, and the polymorphism information content (PIC). It is thought to be a suitable marker for sufficient use with an observation heterozygosity of 0.525, an expected heterozygosity of 0.643, and a PIC of 0.602. As a result of the probabilities of different individuals with the same genotype being found, the random mating population was 4.68 × 10-8 and the half-sib mating population was 3.23 × 10-6. And the non-exclusion probability was analyzed as 0.0516621 and 0.0045074 when there was no information about parents and only information about one parent. Therefore, if it is used as basic data for establishing a production traceability system or improvement of deer through individual identification and paternity test system, it is expected to revitalize the domestic deer industry.