One step purification of flavanone glycosides from Poncirus trifoliata by centrifugal partition chromatography

Abstract

Flavanone glycosides were successfully separated from the crude extract of Poncirus trifoliata by preparative centrifugal partition chromatography with a two-phase solvent system composed of ethyl acetate-acetonitrile-water (3:2:5, v/v/v). Naringin (50.0 mg), neoponcirin (16.8 mg), and poncirin (71.9 mg) were purified from the 524 mg crude extract in only one step. The purities of the isolated compounds were determined to be over 90% by HPLC analysis and their structures were elucidated by 1H NMR, 13C NMR, and ESI-MS. ? 2007 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.