Cohnella cholangitidis sp. nov., a novel species of the genus Cohnella isolated from a clinical specimen in Korea

Abstract

A Gram-positive, aerobic, rod-shaped bacterium, designated as strain 1605-214(T), was isolated from the blood sample of a patient with cholangitis. Based on its 16S rRNA gene sequence, the strain 1605-214(T) belonged to the genus Cohnella and exhibited 97.9% sequence identity with Cohnella luojiensis DSM 24270(T) (GQ214052). DNA-DNA hybridization, digital DNA-DNA hybridization, and average nucleotide identity values between the two species were 23% +/- 1.9, 21.1%, and 77.2%, respectively. The cellular fatty acids of strain 1605-214(T) were mainly comprised of anteiso-C-15:0 (36.1%), iso-C-16:0 (16.5%), and C-16:0 (15.1%). The predominant quinone was menaquinone-7; predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid-1. The cell wall peptidoglycan of strain 1605-214(T) contained meso-diaminopimelic acid. DNA G + C content of strain 1605-214(T) was 50.6 mol%. 5187 genes out of a total of 5413 (94.6%) were assigned putative functions using eggNOG v5.0. Based on genotypic characteristics and genomic sequence analysis results, strain 1605-214(T) was confirmed to represent a novel species of genus Cohnella, for which the name Cohnella cholangitidis sp. nov., was proposed.