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Expression of alpha-galactosidase gene from Leuconostoc mesenteroides SY1 in Leuconostoc citreum
- Issue Date
- Dec-2007
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- alpha-galactosidase; gene expression; Leuconostoc citreum; CCR
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.12, pp 2081 - 2084
- Pages
- 4
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 17
- Number
- 12
- Start Page
- 2081
- End Page
- 2084
- ISSN
- 1017-7825
1738-8872
- Abstract
- A 2.5 kb aga gene encoding alpha-galactosidase (alpha-Gal) from Leuconostoc mesenteroides SY1 was cloned into pSJE, an E. coli-Leuconostoc shuttle vector. The recombinant plasmid, pSJEaga, was introduced into Leuconostoc citreum KCTC3526 (ATCC49370) by electroporation. Transcription level of ago was the highest in cells grown on raffinose (1%, w/v) followed by cells grown on galactose, melibiose, fructose, glucose, and sucrose. Western blot using antibodies against alpha-Gal showed similar results to slot-blot results and enzyme activity measurements. All the results indicated that the ago was successfully expressed in L. citreum and its transcription was under the carbon catabolite repression (CCR).
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