Citicoline reduces upregulated clusterin following kainic acid injection in the rat retina

Abstract

Purpose: To investigate the effects of citicoline on upregulated clusterin and retinal damage induced by kainic acid (KA). Methods: KA was injected into the vitreous of rats. Effects of systemic citicoline treatments were estimated by measuring the thickness of the various retinal layers, immunoblotting, and immunohistochemical techniques. Results: One day after KA injection, the immunoreactivity of clusterin increased significantly. In rats treated with KA plus clusterin immunoreactivity was markedly reduced compared to KA-treated rats. Western blot analysis showed that clusterin protein levels were increased in KA-treated rats, but decreased in KA plus citicoline-treated rats. Apoptotic cell death was determined by TUNEL method. Citicoline reduced the expression of clusterin, as well as the expression of TUNEL after KA injection in the rat retina. Conclusion: The increased expression of clusterin following KA injection in the rat retina suggests the presence of neurodegenerative events; citicoline may provide neuroprotection against neuronal cell damage.