Expression of alpha-amylase gene from Bacillus licheniformis in Lactobacillus brevis 2.14

Abstract

The a-amylase gene, amyL, from Bacillus licheniformis was expressed in Lactobacillus brevis 2.14 and Escherichia coli DH5a using two different shuttle vectors, pCW4 and pSJE. E. coli transformants (TFs) harboring either pCW4Ta or pSJETa produced active a-amylase but L. brevis TFs did not, as determined by enzyme assays and zymography. But amyL transcripts were synthesized in L. brevis TFs. In terms of plasmid stability, pSJE, a theta-type replicon, was more stable than pCW4, an RCR (rolling circle replication) plasmid, in L. brevis without antibiotic selection. Copyright ? 2007 KFN.