Low-density lipoprotein (LDL)-antioxidant lignans from Myristica fragrans seeds

Abstract

Six diarylbutane lignans 1-5 and one aryltetralin lignan 6 were isolated from the methanol (95%) extracts of Myristica fragrans seeds and then 7-methyl ether diarylbutane lignan 4 has proven to be new a compound. Their compounds 1-7 were evaluated for LDL-antioxidant activity to identify the most potent LDL-antioxidant 3 with an IC50 value of 2.6 mu M in TBARS assay. Due to its potency, compound 3 was tested for complementary in vitro investigations, such as lag time (140 min at 1.0 mu M), relative electrophoretic mobility (REM) of ox-LDL (inhibition of 80% at 20 mu M and 72% at 10 mu M), and fragmentation of apoB-100 (inhibition of 93% at 20 mu M) on copper-mediated LDL oxidation. In macrophage-mediated LDL oxidation, the TBARS formation was also inhibited by compound 3. (C) 2007 Elsevier Ltd. All rights reserved.