Crystallization and preliminary crystallographic analysis of decameric and monomeric forms of C49S mutant thioredoxin-dependent AhpC from Helicobacter pylori

Abstract

Cys49Ser mutant Helicobacter pylori alkyl hydroperoxide reductase (C49S HpAhpC) was purified under reducing conditions in monomeric and decameric forms. The monomeric form was crystallized by the hanging-drop vapour-diffusion method. The crystals diffracted to 2.25 angstrom resolution and belonged to space group C2, with unit-cell parameters a = 245.8, b = 140.7, c = 189.5 angstrom, beta = 127 degrees, and contained 20 molecules in the asymmetric unit. A crystal of the decameric form was obtained by the microbatch crystallization method and diffracted to 2.8 angstrom resolution. It belonged to space group C222, with unit-cell parameters a = 257.5, b = 417.5, c = 95.6 angstrom. The structure of the monomeric form of C49S HpAhpC has been solved by the molecular-replacement method.