NF-κB 활성 저해를 통한 대두 유래 Peptide의 Cycooxygenase-2 발현 및 Prostalandin E2 생성의 억제

Abstract

Frequent consumption of soy and soy-based products is associated with reduced inflammation and cancer incidence. Pro-inflammatory mediators, such as prostaglandin E2 (PGE2) and cyclooxygenases-2 (COX-2), play pivotal roles in normal as well as transformed cells. In this study, we examined the effect of peptide [Leu-Leu-Pro-His-His (LLPHH)] derived from soy bean on phorbol 12-myristate 13-acetate (PMA)-induced inflammatory responses using human lymphatic U937 cell model. Treatment of PMA significantly induced COX-2 expression and PGE2 production in U937 cells. However, the pretreatment with LLPHH markedly inhibited the PMA-induced COX-2 expression as well as PGE2 production in a dose-dependent manner. Moreover, the LLPHH prevented the nuclear translocation of nuclear factor kappaB (NF-κB p65) stimulated by PMA treatment. However, the elevated levels of early growth response gene-1 (Egr-1) expression by PAM were not reduced by the pretreatment of LLPHH. Taken together, the present data indicate that the LLPHH derived from soy bean exhibits anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes through the NF-kB signaling pathway. These findings support the hypothesis that the soy peptides reduce the risk of tumorigenesis possibly by suppressing inflammatory responses. (Cancer Prev Res 13, 233-241, 2009)