ACE Inhibitory Lignan Glycosides Isolated from Eucommia ulmoides Oliver

Abstract

To evaluate a potential possibility of Eucommia ulmoides Oliver as a functional food, ACE (angiotensin converting enzyme) inhibitory activities of leaf, bark, stem and 4 compounds isolated from E. ulmoides were tested. The 4 compounds were isolated and purified by silica gel column chromatography, thin layer chromatography and reverse phase column chromatography. Compound I was pinoresinol-4,4'- di-O-β-D-glucoside (PG) and compound Ⅱ was dehydrodiconiferyl alcohol 4,γ'-di-O-β-D-glucopyranoside (DAG) originating from Eucommial Cortex. The highest amount of PG was present at raw and roasted bark as 135.13 mg% and 163.67 mg%, and the highest amount of DAG was present at raw and roasted leaf as 117.93 mg% and 133.93 mg% respectively. In an ACE inhibition test, 10 mg/ml of roasted leaf, raw and roasted bark extracts of E. ulmoides Oliver were 77.49%, 75.72% and 75.36% respectively, and 10mg/ml of PG and DAG were shown to be 78.51 and 81.20% respectively. IC50 values of PG and DAG were 0.6±0.2 and 0.5±0.2 mg/ml respectively.