한우 수정란의 산소 소비량이 수정란이식 수태율에 미치는 영향

Abstract

Oxygen consumption has been regarded as a useful indicator for assessment of mammalian embryo quality. This study was performed to investigate whether oxygen consumption reflects morphological grade of in vivo derived bovine blastocyst-stage embryos (blastocyst). The oxygen consumption of in vitro produced blastocyst was compared to its total cell number. In addition, pregnant rate was measured after transplantation of in vivo blastocysts with different oxygen consumption. The quality of blastocyst collected on day 7 after artificial insemination was categorized as grade Ⅰ and Ⅱ (G Ⅰ and G Ⅱ) based on microscopic observation of the morphology. Oxygen consumption of blastocyst was measured using a scanning electrochemical microscopy (SECM) and total cell number of in vitro blastocyst was enumerated by counting cells stained by propidium iodide. Pregnancy of recipient cow was confirmed with rectal palpation after 60 days of embryo transfer. The oxygen consumptions of G Ⅰ blastocysts were significantly higher than those of G Ⅱ blastocysts (10.2×1015/mol s-1 versus6.4×1015/mol s-1, p<0.05). Total cell numbers of in vitro blastocysts were 74.8, 90.7, and 110.2 in the oxygen consumption of below 10.0, 10.0～12.0, and over 12.0～1015/mol s-1, respectively. Total cell number was significantly increased in embryos with high oxygen consumption (p<0.05). Pregnant rate in recipient cow was 0, 50, and 85.7% in the transplantation of embryo with the oxygen consumption of below 10.0, 10.0～12.0, and over 12.0×1015/mol s, respectively. These results suggest that measurement of oxygen consumption may help increase the pregnant rate of bovine embryos.