APPLICATION OF A SOLID-PHASE FLUORESCENCE IMMUNOASSAY TO DETERMINE AMOXICILLIN RESIDUES IN FISH TISSUE

Abstract

The present study demonstrates an application of Parallux T (a solid-phase fluorescence immunoassay) for amoxicillin analysis in fish tissue. Amoxicillin at the recommended therapeutic dose (400 mg/kg body weight) was orally administered to three groups of 25 olive flounder (Paralichthys olivaceus), 25 rockfish (Sebastes schlegeli) and 25 red sea bream (Pagrus major) for 7 consecutive days. Amoxicillin was detected in the muscle of fish treated by the 3rd day of the withdrawal period. The recovery rates of all spiked muscle samples were >86% of the spiked values. The present study showed that solid-phase fluorescence immunoassay can be easily adopted in predicting amoxicillin residues in the muscle tissue of farmed fish.