Characterization of a profile of the anthocyanins isolated from Vitis coignetiae Pulliat and their anti-invasive activity on HT-29 human colon cancer cells

Abstract

We isolated anthocyanins from fruits of Vitis coignetiae Pulliat, characterized the anthocyanin profile, and investigated the anti-invasive effects of the anthocyanins on human colon cancer cells. The anthocyanins inhibited cell invasion in a dose-dependent manner, as measured by Matrigel invasion assays, by suppression of matrix metalloproteinase (MMP)-2 and MMP-9 expression. The anti-invasive activity of the anthocyanins was associated with modulation of constitutive nuclear factor kappa B (NF-kappa B) activation. The activation of NF-kappa B triggered by tumor necrosis factor-alpha was also inhibited by the anthocyanins through suppression I kappa B alpha phosphorylation. AIMs inhibited the expression of NF-kappa B-regulated proteins. In conclusion, this study suggested that the anthocyanins isolated from fruits of V. coignetiae Pulliat should have anti-invasive activities on human colon cancer cells and the activities should be related to the inhibition of NF-kappa B-regulated proteins such as MMP-2 and MMP-9 expression through the inhibition of NF-kappa B activation. Crown Copyright (C) 2010 Published by Elsevier Ltd. All rights reserved.