Adventitious shoot regeneration from leaf explants of miniature paprika (Capsicum annuum) 'Hivita Red' and 'Hivita Yellow'

Abstract

A simple and efficient protocol was developed for in vitro propagation of two miniature paprika cultivars. Seeds of miniature paprika (Capsicum annuum) 'Hivita Red' and 'Hivita Yellow' were decontaminated and placed in a petri dish containing a half-strength MS medium and then were incubated in the dark for 7 10 days for germination. Leaf explants excised from one month-old aseptic seedlings were cultured on a MS medium supplemented with TDZ (0.1, 0.5, 1.0, 2.0, or 3.0 mgL(-1)) alone or in a combination with NAA (0.1 or 0.01 mgL(-1)) for four weeks. The highest number of regenerated shoot buds was obtained when leaf explants were cultured on a MS medium supplemented with 2.0 mgL(-1) TDZ and 0.1 mgL(-1) NAA with an average shoots per explant of 8.0 in 'Hivita Red' and 5.6 in 'Hivita Yellow'. Regenerated shoot buds were separated and transferred onto a MS medium without growth regulators for shoot growth and rooting. Plantlets were successfully acclimatized in a greenhouse and cultivated for three months. After about two months, they started to produce flowers and continuously produced fruits. Morphology and fruit shape of regenerated plants were normal and plants set seeds as the same as to the seed-raised plants.