NMR study of hydrogen exchange during the B-Z transition of a DNA duplex induced by the Z alpha domains of yatapoxvirus E3L

Abstract

The Yaba-like disease viruses (YLDV) are members of the Yatapoxvirus family and have double-stranded DNA genomes. The E3L protein, which is essential for pathogenesis in the vaccinia virus, consists of two domains: an N-terminal Z-DNA binding domain and a C-terminal RNA binding domain. The crystal structure of the E3L orthologue of YLDV (yabZ alpha(E3L)) bound to Z-DNA revealed that the overall structure of yabZ alpha(E3L) and its interaction with Z-DNA are very similar to those of hZ alpha(ADAR1). Here we have performed NMR hydrogen exchange experiments on the complexes between yabZ alpha(E3L) and d(CGCGCG)(2) with a variety of protein-to-DNA molar ratios. This study revealed that yabZ alpha(E3L) could efficiently change the B-form helix of the d(CGCGCG)(2) to left-handed Z-DNA via the active-mono B-Z transition pathway like hZ alpha(ADAR1). (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.