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Molecular cloning and antiviral activity of IFN-beta promoter stimulator-1 (IPS-1) gene in Japanese flounder, Paralichthys olivaceus

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dc.contributor.authorSimora, Rhoda Mae C.-
dc.contributor.authorOhtani, Maki-
dc.contributor.authorHikima, Jun-ichi-
dc.contributor.authorKondo, Hidehiro-
dc.contributor.authorHirono, Ikuo-
dc.contributor.authorJung, Tae Sung-
dc.contributor.authorAoki, Takashi-
dc.date.accessioned2022-12-27T04:02:57Z-
dc.date.available2022-12-27T04:02:57Z-
dc.date.issued2010-12-
dc.identifier.issn1050-4648-
dc.identifier.issn1095-9947-
dc.identifier.urihttps://scholarworks.gnu.ac.kr/handle/sw.gnu/24848-
dc.description.abstractThe mitochondrial adaptor, IFN-beta promoter stimulator-1 (IPS-1), also known as MAVS/VISA/Cardif, plays a key role in the signal transduction of the RIG-1/MDA5 pathway to induce the production of interferons (IFNs) and other cytokines. In the present study, Japanese flounder (Paralichthys olivaceus) IPS-1 cDNA was cloned from Japanese flounder spleen using PCR-based methods. The full-length cDNA has 2235 nucleotides and encodes a polypeptide of 641 amino acids. The putative Japanese flounder IPS-1 protein contains an N-terminal CARD-like domain, a central proline-rich domain, a C-terminal transmembrane domain, and exhibits similarity to other teleost counterparts ranging from 20% to 34%. Semi-quantitative RT-PCR showed that Japanese flounder IPS-1 mRNA was expressed in all tissues examined. The expression level of flounder IPS-1 gene was unchanged in viral hemorrhagic septicemia virus (VHSV)-infected kidney as measured by quantitative real-time PCR (Q-PCR). In addition, Japanese flounder IPS-1-overexpressing cells were protected against hirame rhabdovirus (HIRRV) and VHSV infection as manifested by the delayed appearance of cytopathic effect (CPE) and decreased viral titers. Expression of IFN-inducible genes including Mx, ISG15 and IRF3 were also induced in the IPS-1-overexpressing cells. These results suggest that Japanese flounder IPS-1 is involved in the antiviral immunity as a one of the adaptors in fish IFN-activation pathway. (C) 2010 Elsevier Ltd. All rights reserved.-
dc.format.extent8-
dc.language영어-
dc.language.isoENG-
dc.publisherAcademic Press-
dc.titleMolecular cloning and antiviral activity of IFN-beta promoter stimulator-1 (IPS-1) gene in Japanese flounder, Paralichthys olivaceus-
dc.typeArticle-
dc.publisher.location영국-
dc.identifier.doi10.1016/j.fsi.2010.08.012-
dc.identifier.scopusid2-s2.0-77957751161-
dc.identifier.wosid000284798400010-
dc.identifier.bibliographicCitationFish and Shellfish Immunology, v.29, no.6, pp 979 - 986-
dc.citation.titleFish and Shellfish Immunology-
dc.citation.volume29-
dc.citation.number6-
dc.citation.startPage979-
dc.citation.endPage986-
dc.type.docTypeArticle-
dc.description.isOpenAccessN-
dc.description.journalRegisteredClasssci-
dc.description.journalRegisteredClassscie-
dc.description.journalRegisteredClassscopus-
dc.relation.journalResearchAreaFisheries-
dc.relation.journalResearchAreaImmunology-
dc.relation.journalResearchAreaMarine & Freshwater Biology-
dc.relation.journalResearchAreaVeterinary Sciences-
dc.relation.journalWebOfScienceCategoryFisheries-
dc.relation.journalWebOfScienceCategoryImmunology-
dc.relation.journalWebOfScienceCategoryMarine & Freshwater Biology-
dc.relation.journalWebOfScienceCategoryVeterinary Sciences-
dc.subject.keywordPlusNF-KAPPA-B-
dc.subject.keywordPlusRIG-I-
dc.subject.keywordPlusSIGNALING PROTEIN-
dc.subject.keywordPlusADAPTER PROTEIN-
dc.subject.keywordPlusINNATE IMMUNITY-
dc.subject.keywordPlusRECOGNITION-
dc.subject.keywordPlusVIRUS-
dc.subject.keywordPlusRNA-
dc.subject.keywordPlusINDUCTION-
dc.subject.keywordPlusIDENTIFICATION-
dc.subject.keywordAuthorJapanese flounder-
dc.subject.keywordAuthorMitochondrial adaptor-
dc.subject.keywordAuthorIPS-1 (MAVS)-
dc.subject.keywordAuthorHIRRV-
dc.subject.keywordAuthorVHSV-
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