TLC 분석에 의한 산청지역의 녹차 채엽시기에 따른 β-Carotene과 Lutein 함량의 변화

Abstract

This research was conducted to produce high quality green tea during different harvesting times at Sanchung by TLC assay and to prevent the loss of chlorophyll and carotenoid contents. In a comparison of different harvesting periods, the 3rd harvesting green tea (3rd GT) contained much higher total chlorophyll content than the 1st harvesting green tea (1st GT) and 2nd harvesting green tea (2nd GT). To isolate both β-carotene and lutein in green tea by TLC assay, the ratio of n-hexane and acetone in the development solvent was 50 to 50 (v/v) after developed as 85 to 15. Their isolation was also satisfactory as two-dimensional chromatography on one adsorbent combined development of the plate in the first direction with 40 to 20 and in the second direction perpendicular to the first one with 50 to 50 (v/v). Regarding β-carotene content per dry weight, the 2nd GT contained more than both the 1st GT and 3rd GT, whereas regarding per total chlorophyll, the 1st GT contained more than both the 2nd GT and 3rd GT. Regarding content of lutein, the 1st GT contained more than the 2nd GT and 3rd GT in both per dry weight and per total chlorophyll. Both the effect of inhibition on bacterial growth and the survival test of Artemia using green tea extract showed optimal conditions at a concentration of 200 μg mL^-1.