Efficiency of shoot regeneration from leaf, stem, petiole and petal explants of six cultivars of Chrysanthemum morifolium

Abstract

Leaf, petal, petiole, and intermodal stems of six chrysanthemum cultivars were incubated on a medium supplemented with 0.00-6.66 mu M benzyladenine (BA) either alone or in combination with 2.85-8.56 mu M indoleacetic acid (IAA), and 0.46 mu M kinetin (Kin). Significant differences in frequency of regeneration were observed among different cultivars when grown on media containing different plant growth regulators (PGRs). The highest frequency of regeneration was observed for cv. Brighton followed by cvs. Dark Roanoke and Tahoe. Low frequencies of shoot organogenesis were observed for cvs. Dark Orange Cupertino, Hudson Bay, and Ivory Eugene. Among different explants, petals exhibited the highest frequencies of shoot organoenesis and mean number of shoots per explant. Regenerated shoots of cv. Brighton transferred onto a medium containing 2.85-8.56 mu M IAA developed highest frequency of roots (100%) with a mean of 7.6 roots per shoot. Well-rooted shoots transferred to plastic pots containing a soil mix were kept in a growth room for 1 week, and then moved to the greenhouse for further growth and development.