Construction of a conditional lethal Salmonella mutant via genetic recombination using the ara system and asd gene
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Sam Woong | - |
dc.contributor.author | Kang, Ho Young | - |
dc.contributor.author | Hur, Jin | - |
dc.contributor.author | Gal, Sang Wan | - |
dc.contributor.author | Bang, Woo Young | - |
dc.contributor.author | Cho, Kwang-Keun | - |
dc.contributor.author | Kim, Chul Wook | - |
dc.contributor.author | Bahk, Jeong Dong | - |
dc.contributor.author | Lee, John Hwa | - |
dc.date.accessioned | 2022-12-27T02:52:09Z | - |
dc.date.available | 2022-12-27T02:52:09Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2011-11 | - |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/23495 | - |
dc.description.abstract | In order to construct a conditional lethal Salmonella mutant, an arabinose-regulated recombinant genetic system was used. The Salmonella aspartate semialdehyde dehydrogenase (asd) gene was localized under the control of araC P-araBAD in a plasmid to create the araC P-araBAD::asd cassette. The cassette was cloned into a plasmid carrying a p15A replication origin to create the recombinant plasmid pMMP55. The growth of Salmonella MMP10 harboring pMMP55 was dependent on the presence of arabinose. In the presence of arabinose, the Asd deficiency due to chromosomal deletion of asd in the Salmonella host was complemented by the asd gene transcribed and translated under the P-araBAD promoter and araBAD Shine-Dalgarno (SD) sequence in pMMP55. Growth inhibition of the strain was demonstrated by arabinose depletion in M9 minimal medium, indicating that the strain were unable to grow in an arabinose-limited environment. In addition, the analysis of a 50% lethal dose (LD50) using mice revealed that the strain MMP10 exhibited attenuation by approximately 100-fold relative to that of the unmodified strain. In conclusion, these data suggest that the araC P-araBAD::asd system developed in this study can be used to construct conditional lethal Salmonella mutants for application as safe, live-attenuated Salmonella vaccines. (C) 2011 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | LIVE ATTENUATED SALMONELLA | - |
dc.subject | ESCHERICHIA-COLI | - |
dc.subject | LEADER PEPTIDASE | - |
dc.subject | PLASMID DNA | - |
dc.subject | PROTEIN | - |
dc.subject | ENTERICA | - |
dc.subject | TRANSFORMATION | - |
dc.subject | TYPHIMURIUM | - |
dc.subject | VACCINATION | - |
dc.subject | PERSISTENCE | - |
dc.title | Construction of a conditional lethal Salmonella mutant via genetic recombination using the ara system and asd gene | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Gal, Sang Wan | - |
dc.contributor.affiliatedAuthor | Cho, Kwang-Keun | - |
dc.identifier.doi | 10.1016/j.mimet.2011.08.004 | - |
dc.identifier.scopusid | 2-s2.0-80053574872 | - |
dc.identifier.wosid | 000296822300013 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGICAL METHODS, v.87, no.2, pp.202 - 207 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.citation.title | JOURNAL OF MICROBIOLOGICAL METHODS | - |
dc.citation.volume | 87 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 202 | - |
dc.citation.endPage | 207 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | LIVE ATTENUATED SALMONELLA | - |
dc.subject.keywordPlus | ESCHERICHIA-COLI | - |
dc.subject.keywordPlus | LEADER PEPTIDASE | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | PROTEIN | - |
dc.subject.keywordPlus | ENTERICA | - |
dc.subject.keywordPlus | TRANSFORMATION | - |
dc.subject.keywordPlus | TYPHIMURIUM | - |
dc.subject.keywordPlus | VACCINATION | - |
dc.subject.keywordPlus | PERSISTENCE | - |
dc.subject.keywordAuthor | Conditional lethal mutant | - |
dc.subject.keywordAuthor | asd | - |
dc.subject.keywordAuthor | Salmonella | - |
dc.subject.keywordAuthor | Vaccine | - |
dc.subject.keywordAuthor | ara operon | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Gyeongsang National University Central Library, 501, Jinju-daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea+82-55-772-0533
COPYRIGHT 2022 GYEONGSANG NATIONAL UNIVERSITY LIBRARY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.