Resveratrol Reduces TNF-alpha-induced U373MG Human Glioma Cell Invasion through Regulating NF-kappa B Activation and uPA/uPAR Expression

Abstract

Background: High invasiveness of glioma cells is one of the reasons that patients with malignant glioma have a poor prognosis. Resveratrol, a plant compound abundant in the peel of grapes, has been suggested as a potential cancer chemopreventive agent. Therefore, we investigated the effect of resveratrol on glioma cell invasion. Materials and Methods: The effect of resveratrol on U373MG human glioma cell invasion was assessed by Matrigel assay and tnethylthiazoltetrazolium assay. Western blotting and reverse transcription-polymerase chain reaction were performed to elucidate the action mechanism of resveratrol. Results: Resveratrol reduced tumor necrosis factor (TNF)-alpha-induced U373MG human glioma cell invasion. In addition, resveratrol repressed nuclear factor kappa B (NF-kappa B) activation and down-regulated mRNA expression of urokinase plastninogen activator (uPA) and its receptor in TNF-alpha-treated glioma cells. Conclusion: These findings suggest that resveratrol could prevent glioma cell invasion via inhibiting proteolysis of extracellular matrix.