Anti-inflammatory effect of flavonoids isolated from Korea Citrus aurantium L. on lipopolysaccharide-induced mouse macrophage RAW 264.7 cells by blocking of nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signalling pathways
  • Kang, Sang Rim
  • Park, Kwang Il
  • Park, Hyeon Soo
  • Lee, Do Hoon
  • Kim, Jin A.
  • ... Lee, Won Sup
  • 외 6명
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초록

Citrus fruits are an abundant source of various flavonoids, which have been used as a traditional herbal medicine in Korea and China. Most flavonoids are known to have anti-oxidant, anti-bacterial and analgesic properties. In this study, it was examined whether flavonoids (nobiletin, naringin and hesperidin) isolated from Korea Citrus aurantium L inhibited the pro-inflammatory mediators including cytokines by blocking nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signalling in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. The flavonoids suppressed mRNA and protein expression of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in LPS-induced macrophages. The molecular mechanism was associated with inhibition of the degradation/phosphorylation of I-kappa B-alpha and nuclear translocation of the NF-kappa B p-65 as well as phosphorylation of MAPK by flavonoids. These results suggest that flavonoids have anti-inflammatory effects by suppressing expression of COX-2, iNOS and cytokines by blocking the NF-kappa B and MAPK signalling in RAW 264.7 macrophages. (C) 2011 Elsevier Ltd. All rights reserved.

키워드

Citrus aurantium L.Anti-inflammationCyclooxygenase-2Inducible nitric oxide synthaseNuclear factor-kappa B (NF-kappa B)Mitogen-activated protein kinase (MAPK)NITRIC-OXIDE PRODUCTIONCYCLOOXYGENASE-2 EXPRESSIONCOX-2 EXPRESSIONINHIBITIONSYNTHASEP38ATTENUATIONEXTRACTALPHAINOS
제목
Anti-inflammatory effect of flavonoids isolated from Korea Citrus aurantium L. on lipopolysaccharide-induced mouse macrophage RAW 264.7 cells by blocking of nuclear factor-kappa B (NF-kappa B) and mitogen-activated protein kinase (MAPK) signalling pathways
저자
Kang, Sang RimPark, Kwang IlPark, Hyeon SooLee, Do HoonKim, Jin A.Nagappan, ArulkumarKim, Eun HeeLee, Won SupShin, Sung ChulPark, Moon KiHan, Dae YongKim, Gon Sup
DOI
10.1016/j.foodchem.2011.06.039
발행일
2011-12-15
유형
Article
저널명
Food Chemistry
129
4
페이지
1721 ~ 1728