저농도 Diethylnitrosamine으로 위장 내 이형성 병변 유도

Abstract

Gastric cancer was one of the most common cancers in the world. However, only a few animal models are available for this disease, especially for the precancerous lesions. Nitrosamines are known as carcinogen and have a potential to induce cancer in the digestive tract. This study was to determine whether a low concentration of diethylnitrosamine (DEN) induced precancerous lesions or cancer of the stomach without serious side effects. Ten Sprague Dawely rats were randomly assigned to two groups which administered either water (G1), 0.01% DEN-containing water (G2) for a period of 42 weeks. Mice were sacrificed; their stomachs and livers were sectioned and stained with hematoxylin/eosin. Stomachs were analyzed by a pathologist for gastric and hepatic lesions. Ten specimens were studied. No statistical difference in weights of whole bodies and the liver were observed between the two groups (p＞0.05). In 42 weeks, focal lesions with dysplasia were more frequently seen in G2. However, no gastric cancer was observed in G2. In G2, there were no significant side effects of DEN except mild fat infiltration around porta hepatis. In conclusion, this study suggests that 42-week-ingestion of DEN (10 mg/l) can induce small focal dysplastic lesions in the stomach without serious side effects, but that the 42-week-ingestion of DEN is not enough to develop overt gastric cancer in Sprague Dawely rats.