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Crystallization and preliminary X-ray diffraction analysis of mevalonate kinase from Methanosarcina mazeiopen access
- Issue Date
- Dec-2012
- Publisher
- International Union of Crystallography
- Keywords
- Methanosarcina mazei; mevalonate kinase; multiple-wavelength anomalous dispersion
- Citation
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications, v.68, no.12, pp 1560 - 1563
- Pages
- 4
- Indexed
- SCIE
SCOPUS
- Journal Title
- Acta Crystallographica Section F: Structural Biology and Crystallization Communications
- Volume
- 68
- Number
- 12
- Start Page
- 1560
- End Page
- 1563
- ISSN
- 1744-3091
2053-230X
- Abstract
- Mevalonate kinase (MVK), which plays an important role in catalysing the biosynthesis of isoprenoid compounds derived from the mevalonate pathway, transforms mevalonate to 5-phosphomevalonate using ATP as a cofactor. Mevalonate kinase from Methanosarcina mazei (MmMVK) was expressed in Escherichia coli, purified and crystallized for structural analysis. Diffraction-quality crystals of MmMVK were obtained by the vapour-diffusion method using 0.32 M MgCl2, 0.08 M bis-tris pH 5.5, 16%(w/v) PEG 3350. The crystals belonged to space group P2(1)2(1)2, with unit-cell parameters a = 97.11, b = 135.92, c = 46.03 angstrom. Diffraction data were collected to 2.08 angstrom resolution.
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