Localization and Expression of TREK-1, TREK-2, and TRAAK Channels in Rat Medial Vestibular Nuclei

Abstract

Objective: The aim of this study was to examine the localization and expression of TREK-1, TREK-2 and TRAAK channels in rat medial vestibular nuclei by immunohistochemical staining, reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blot analysis. Materials and Methods: Twenty eight male Sprague-Dawley rats were used in this study. Antibodies for TREK-1, TREK-2 and TRAAK channels were used. Tissues containing medial vestibular nuclei were selectively for RT-PCR and Western blot analysis. Results: TREK-1, TREK-2 and TRAAK channels immunopositive neurons were distributed throughout the medial vestibular nuclei. RT-PCR data showed TREKs/TRAAK mRNAs were expressed in the medial vestibular nuclei. Western blot results also showed TREKs/TRAAK protein expression in the medial vestibular nuclei. Conclusion: This study may provide additional speculation into the role of K2p channels in the pathway between migraine and vestibular system.