토양 중 고농도 카드뮴에 노출된 MuS1 형질전환 담배 (Nicotiana tabacum cv. Xanthi)의 생리적 반응 및 카드뮴 축적: 식물학적 오염토양정화를 위한 형질전환 식물 탐색

Abstract

The objective of this study was to understand the physiological response and cadmium accumulation of MuS1transgenic tobacco exposed to high concentration of Cd in soil. For this, a pot experiment was carried out in a greenhouse for a month, with two lines of MuS1 transgenic tobaccos (S4 and S6) and non-transgenic tobacco cultivated in the soils spiked at three different Cd concentrations (0, 60 and 180 mg kg -1). Both transgenic and non-transgenic tobacco showed visible toxic symptoms such as chlorosis and leaf roll as treated concentration increased. The net photosynthetic rates of MuS1 plants (S4 and S6) exposed at 180 mg kg -1Cd were 6.3 and 7.7μmol m -2s-1, being higher than those of the non-transgenic plant (4.8 μmol m -2s-1). Values of stomatal conductance of MuS1 transgenic plants (0.05 and 0.008 mmol H2O m -2s-1) were also higher than those of non-transgenic plant (0.03 mmol H2O m -2s-1). In addition, fresh and dry weights of MuS1 transgenic plants were heavier than those of non-transgenic plant. Likewise, MuS1 transgenic plants appeared to be better physiological performance than non-transgenic tobacco when exposed at high concentration of Cd in soil. With regard to metal accumulation, MuS1 transgenic tobaccos accumulated more Cd in their roots than non-transgenic tobacco implying that MuS1 transgenic tobacco is suggested to be used for phytostabilization of heavy metals.