Osteoblastic Response to the Hydroxyapatite/Gelatin Nanocomposite and Bio-Calcium Phosphate Cement

Abstract

The main purpose of the developingnew CPCs by modulating their components or ratios are to develop more proper biomaterials which can adapt to the body. In the present study, the properties of newly developed HAp/Gel Nanocomposites were tested using in-vitro experiments (The toxicity test, chromosomal aberration test, cytokinesis-block micronucleus assay, RT-PCR analysis). The data from the WST-1 experiments using HAp/Gel and human osteoblastic cells showed that this material does not induces cellular death. And this material did not induced any significant changes in the structures of chromosomes. The expression pattern of integrins after treatment with CPC or HAp/Gel powder represents that the cells in CPC forms focal adhesion as the cells on adhesive culture dish, but the cells in HAp/Gel do not. By performing several set of experiments, HAp/Gel is not toxic to hFOB-1.19 human osteoblast cell and does not cause any genetic problems. In contrast to CPC, HAp/Gel promotes the final differentiation of osteoblast into osteocyte. These results represent the improved bio-mimetic properties of HAp/Gel.