Heat shock protein profiles on the protein and gene expression levels in olive flounder kidney infected with Streptococcus parauberis

Abstract

Heat shock proteins (HSPs) have been observed in cells exposed to a variety of stresses, including infectious pathogens. This study used a label-free, quantitative proteomic approach and transcriptional gene expression analysis to investigate infection-related HSP proteins and their encoding genes in whole kidneys from olive flounder (Paralichthys olivaceus). During Streptococcus parauberis infection in the flounder, the genes encoding Hsp10, Hsp40A4, Hsp40B6, Hsp40B11, Hsp60, Hsp70, glucose regulated protein 78 (Grp78), Hsp90 alpha, Hsp90 beta and Grp94 were induced, and the protein levels of Hsp60, Hsp70, Hsp90 alpha, Hsp90 beta and Grp94 were differentially regulated over time. Subsequent results also revealed that Hsp60, Hsp70, Hsp90 alpha, Hsp90 beta and Grp94 appear to be the dominant and critical HSPs in olive flounder during bacterial infection. This is the first estimation of the differential involvement of HSPs in the immune response of olive flounder exposed to bacterial infection. (C) 2013 Elsevier Ltd. All rights reserved.