과배란 처리에 있어 성감별 정액을 이용한 한우 체내 수정란의 생산 효율

Abstract

Sexed semen is commonly used for the production of calves of the desired gender. Gender selection is importantin animal production industries. For example, female cattle are required for the dairy industry while males are preferredin the beef cattle industry. The present study was to assess the in vivo embryo production efficiency using the semenseparated according to sex during superovulation in Hanwoo. Seventy Hanwoo donor cows were flushed on day 7of estrus cycle with same FSH and artificial insemination by the same technicians. Embryos were recovered on 7 daysafter the third insemination by flushing the uterus with embryo collection medium. KPN semen straws used artificialinsemination contained 20 million sperm (total number 60 million per donor). Sex-sorted semen straws contained 4million sperm (total number 12 million per donor). The results obtained were as follows: No differences were observedin the efficiency of superovulation rates on KPN semen 87%, and sexed semen 100%, respectively. The mean numbersof total embryos are each 12.58 ± 8.31 and 13.25 ± 7.86. The mean numbers of transferable embryos, sexed semenwere significantly lower than KPN semen (3.75 ± 1.98 vs. 8.23 ± 6.07, P<0.05). The rates of unfertilized embryos fromsuperovulation using sexed semen were significantly higher than KPN semen (50% vs. 15%, P<0.05). The rate ofdegenerated 2-cell embryos from sexed and KPN semen was 60.87% and 11.11%, respectively (p<0.05). In conclusion,these results indicate that superovulation using sexed semen was useful, but efficient embryo production was importantto reducing the damage caused by the Flowcytometer-based sperm sorting procedure