OPU 채란계절이 한우의 난자 품질 및 발달 능력에 미치는 영향

Abstract

Implementation of smart embryo technologies in cattle e.g. ovum pick-up followed by in vitro embryo production(OPU-IVP). Seasonal variation is important factor for follicular growth, oocytes quality, quantity and developmentalcompetence. Therefore the aim of present study was carried out to investigated whether the seasons (hot and cool) effecton follicular development, oocyte recovery and subsequent embryo development. Follicular oocytes were aspirated fromKorean native cows (Hanwoo) by the ovum pick-up (OPU) method, which was performed 24 times during two differentseasons, the hot (July to September) and cool (October to December), from OPU donors. The recovered oocytes wereclassified according to morphological categories and used for in vitro embryo production (IVEP). The mean numberof total follicles was significantly higher (p<0.05) during the hot season (18.32 ± 2.26) compared to cool season (15.41± 3.34). Furthermore, seasons did not significantly effect on the number of oocytes recovered (hot season: 41.16%vs. cool season: 46.14%). However, the average number of Grade A oocytes was significantly greater during hot (1.75± 1.86) season compared to the cool season (1.00 ± 1.46), but there was no significant difference of other gradesoocytes. The cleavage rate (hot: 66.67% vs. cool: 63.3%) and embryo development (hot: 58.95% vs. cool: 56.97%)did not differ significantly between the seasons. In conclusion, the results of present study suggest that the season(hot and cool) does not have effects on the oocyte recovery and embryo developmental competence of in vitro culturedembryos.