Ortho-phenylphenol을 주성분을 하는 훈증소독제의 Clostridium perfringens 아포에 대한 살아포 효과

Abstract

This study was performed to evaluate the sporicidal efficacy of a fumigation disinfectant containing20% ortho-phenylphenol against Clostridium perfringens (C. perfringens) spores. In this research, efficacy test offumigant against C. perfringens spores was carried out according to French standard NF T 72-281. C. perfringensspores working culture suspension number (N value), all the spore numbers on the carriers exposed with the fumigant(n1, n2, and n3), the number of bacterial spore suspensions by pour plate method (N1), the number of bacterial sporesuspensions by filter membrane method (N2) and the mean number of bacterial spore recovered on the control-carriers(T value) were obtained from the preliminary test. In addition, the reduction number of C. perfringens spores exposedwith the fumigant (d value) was calculated using T value, the mean number of bacterial spore in recovery solution(n'1) and the mean number of bacterial spore on carriers plated in agar (n'2). N value was 4.3 × 107 spores/mL, and n1,n2, and n3 were higher than 0.5N1, 0.5N2 and 0.5N1, respectively. Additionally, T value was 4.9 × 105 spores/carrier. In the sporicidal effect of the fumigant, the d value was 4.52log reduction. According to the French standard for thefumigant, the d value for the effective sporicidal fumigant should be over than 3log reduction. The results indicatedthat Fumagari OPP® had an efficient sporicidal activity against spores of C. perfringens, then the fumigant can beapplied to disinfect food materials and kitchen appliances contaminated with bacterial spores.