Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis
- Authors
- Lee, Jin Ju; Lim, Jeong Ju; Kim, Dae Geun; Simborio, Hannah Leah; Kim, Dong Hyeok; Reyes, Alisha Wehdnesday Bernardo; Min, WonGi; Lee, Hu Jang; Kim, Dong Hee; Chang, Hong Hee; Kim, Suk
- Issue Date
- 4-Sep-2014
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Brucella abortus; Culture supernatant proteins; Virulence; Protection
- Citation
- COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES, v.37, no.4, pp.221 - 228
- Indexed
- SCIE
SCOPUS
- Journal Title
- COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
- Volume
- 37
- Number
- 4
- Start Page
- 221
- End Page
- 228
- URI
- https://scholarworks.bwise.kr/gnu/handle/sw.gnu/18790
- DOI
- 10.1016/j.cimid.2014.06.001
- ISSN
- 0147-9571
- Abstract
- In this study, we characterized the secreted proteins of Brucella abortus into the enriched media under the bacterial laboratory growth condition and investigated the pathogenic importance of culture supernatant (CS) proteins to B. abortus infection. CS proteins from stationary phase were concentrated and analyzed using 2D electrophoresis. In MALDI TOF/TOF analysis, more than 27 proteins including CuZn SOD, Dps, Tat, OMPs, Adh, LivF, Tuf, SucC, GroEL and DnaK were identified. Cytotoxic effects of CS proteins were found to increase in a dose-dependent manner in RAW 264.7 cells. Upon B. abortus challenge into phagocytes, however, CS proteins pre-treated cells exhibited lower bacterial uptake and intracellular replication compared to untreated cells. Immunization with CS proteins induced a strong humoral and cell mediated immune responses and exhibited significant higher degree of protection against virulence of B. abortus infection compared to mice immunized with Brucella broth protein (BBP). Taken together, these results indicate that B. abortus secreted a number of soluble immunogenic proteins under laboratory culture condition, which can promote antibody production resulted in enhancing host defense against to subsequently bacterial infection. Moreover, further analysis of CS proteins may help to understand the pathogenic mechanism of B. abortus infection and host-pathogen interaction. (C) 2014 Elsevier Ltd. All rights reserved.
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Collections - 농업생명과학대학 > 축산과학부 > Journal Articles
- College of Medicine > Department of Medicine > Journal Articles
- 수의과대학 > Department of Veterinary Medicine > Journal Articles
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