복분자(Rubus coreanus) 추출물의 라디칼 소거활성 및 고지방 식이에 대한 흰쥐의 간 지질 개선 효과

Abstract

This study was investigated the effects on the radical scavenging activities and hepatic lipid levels in rats fed a high-fat diet (HFD) by the 70% ethanolic extract from Rubus coreanus. The Rubus coreanus extract (RCE) was administered through the gastrointestinal tract at 150 mg/kg BW/day for 5 weeks (HFD+RCE). Total phenol content was 157.93 mg gallic acid/g in the RCE. Scavenging activities to DPPH, ABTS, an d hydroxyl radicals by RCE significantly increased in a does-dependent manner of 50∼500 g/mL concentration. DPPH and ABTS radical scavenging activities were over the 70% in the 500 g/mL concentration. During the five weeks study, the food efficiency ratio was no significant dif-ferences between the HFD and HFD+RCE. Liver weight of HFD+RCE (3.75 g/100 g BW) was sig-nificantly decreased compared to those of the HFD. Hepatic triglyceride and total cholesterol levels of HFD+RCE were significantly lower compared to those of the HFD. The hepatic TBARS contents in the HFD+RCE was significantly decreased to the HFD, radical scavenging activity was markedly in-creased in HFD+RCE as normal group. In histological observation of liver tissues, the hepatocytes of HFD+RCE appeared the reduced aspects on the size and numbers of lipid droplets in cytoplasm com-pared to the HFD. Our results suggest that RCE could have antioxidant activities and hepatic hypolipi-demic effects by its phenolic compounds.