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Use of a triiodide resin for isolation of axenic cultures of microalgal Nannochloropsis gaditana
- Issue Date
- Sep-2015
- Publisher
- ELSEVIER SCI LTD
- Keywords
- Microalgae; Triiodide resin; Axenic culture; Nannochloropsis gaditana
- Citation
- BIORESOURCE TECHNOLOGY, v.191, pp 391 - 394
- Pages
- 4
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- BIORESOURCE TECHNOLOGY
- Volume
- 191
- Start Page
- 391
- End Page
- 394
- ISSN
- 0960-8524
1873-2976
- Abstract
- Triiodide resin (TR) was used to generate axenic cultures of microalgae by employing the antibacterial capability of triiodide. A Nannochloropsis gaditana culture contaminated with bacteria was passed through a column filled with TR using the gravity flow. Based on analyses of flow cytometry and vital staining using a fluorescent dye SYTOX Green, three cycles of TR treatments remarkably reduced the number of viable bacteria but had little effects on the microalgae. This novel approach is a simple, rapid, and cost-effective method that can be used to isolate axenic cultures of microalgae. (C) 2015 Elsevier Ltd. All rights reserved.
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