반응표면 분석법에 의한 흑마늘 추출물이 첨가된 간장의 제조 조건 설정

Abstract

Purpose: The central composition design was used to optimize the mixture conditions of black garlic extract. Methods: The response surface methodology (RSM) was carried out from concentration of black garlic extract (X1) and the amount of the black garlic extract (X2) as independent variables, and salts (Y1), reducing sugars (Y2), the content of total phenolic compounds (Y3) and ABTS radical scavenging activity (Y4) as dependent variables. We confirmed the conditions that salinity was minimized and reducing sugar, total phenolic compounds and ABTS radical scavenging activity had maximum values through the response surface analysis. Results: All results had saddle points in originally set up conditions hence, ridge analysis was carried out for narrowing the experimental area. The minimum salt concentration was 16.03% at black garlic extract concentration of 14.84 °brix and contents of 9.26%. Reducing sugar content had maximum of value 7.30 g/mL at 24.58 °brix and contents of 8.08%. Total phenolic compounds contents and ABTS radical scavenging activity had maximum values at black garlic extract concentration of 20.33 and 25.02 °brix. The results indicate that addition of black garlic extract contributed to increased reducing sugar, phenolic compounds contents and antioxidant activity of the soy sauce, but the salt concentration was not significantly affected. Conclusion: Based on the results of RSM, the optimum ranges of addition conditions for lowering the salt concentration and, increasing the sensory and functional ability of soy sauce were as follows: black garlic extract concentration of 15-25 °brix and content of 7.8-9.3%.