자몽종자추출물, 구연산, 사과산 그리고 염화벤잘코늄을 주성분으로 하는 스프레이형 소독제의 조류인플루엔자바이러스에 대한 살바이러스 효과

Abstract

Objectives: This study evaluated the virucidal efficacy against avian influenza virus (AIV) of a disinfectantspray containing 0.25% grapefruit seed extract, 0.2% citric acid, 0.0625% malic acid and 0.0125% benzalkoniumchloride. Methods: The disinfectant spray was diluted several times with hard water (HW) and organic matter (OM). Twopoint five mL of each diluent was added into each test tube, and 2.5 mL of AIV suspension was inserted intoeach test tube. After 30 minutes of virus-disinfectant contact reaction at 4oC, 2.5 mL of 10% inactivated fetalbovine serum was added into each test tube to neutralize the sanitizer efficacy. The neutralized solutions wereserial 10-fold dilutions with phosphate buffer solution, and 0.2 mL of the diluents was injected into the allantoiccavity of five ten-day-old-chickens per dilution time. After incubation of the embryos for five days, the viabilityof the AIV was examined by hemagglutination titer. The valid dilution of the disinfectant spray was estimatedaccording to the dilution time that the virus titer was inactivated more than 104 50% egg-infective dose (EID50)/mL compared with pathogen control. Results: In HW and OM conditions, the valid dilutions of the disinfectant spray against AIV were seven- and three-fold dilutions, respectively. The AIV titer of the pathogen control was more than 6.1 log10EID50/mL, and there was no embryonic toxicity. Conclusion: The present study showed that this disinfectant spray has effective virucidal activity against AIV.