Identification of proteins in hyperglycemia and stroke animal models
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sung, Jin-Hee | - |
dc.contributor.author | Shah, Fawad-Ali | - |
dc.contributor.author | Gim, Sang-Ah | - |
dc.contributor.author | Koh, Phil-Ok | - |
dc.date.accessioned | 2022-12-26T20:31:16Z | - |
dc.date.available | 2022-12-26T20:31:16Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 0022-4804 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/15762 | - |
dc.description.abstract | Background: Stroke is a major cause of disability and death in adults. Diabetes mellitus is a metabolic disorder that strongly increases the risk of severe vascular diseases. This study compared changes in proteins of the cerebral cortex during ischemic brain injury between nondiabetic and diabetic animals. Methods: Adult male rats were injected with streptozotocin (40 mg/kg) via the intraperitoneal route to induce diabetes and underwent surgical middle cerebral artery occlusion (MCAO) 4 wk after streptozotocin treatment. Cerebral cortex tissues were collected 24 h after MCAO and cerebral cortex proteins were analyzed by two-dimensional gel electrophoresis and mass spectrometry. Results: Several proteins were identified as differentially expressed between nondiabetic and diabetic animals. Among the identified proteins, we focused on the following metabolism-related enzymes: isocitrate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, adenosylhomocysteinase, pyruvate kinase, and glucose-6-phosphate isomerase (neuroleukin). Expression of these proteins was decreased in animals that underwent MCAO. Moreover, protein expression was reduced to a greater extent in diabetic animals than in nondiabetic animals. Reverse transcription-polymerase chain reaction analysis confirmed that the diabetic condition exacerbates the decrease in expression of metabolism-related proteins after MCAO. Conclusions: These results suggest that the diabetic condition may exacerbate brain damage during focal cerebral ischemia through the downregulation of metabolism-related proteins. (C) 2016 Elsevier Inc. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | S-ADENOSYLHOMOCYSTEINE HYDROLASE | - |
dc.subject | CEREBRAL-ARTERY OCCLUSION | - |
dc.subject | ISOCITRATE DEHYDROGENASE | - |
dc.subject | GLUCOSE-6-PHOSPHATE ISOMERASE | - |
dc.subject | GLUCOSE DEPRIVATION | - |
dc.subject | OXIDATIVE STRESS | - |
dc.subject | INFARCT VOLUME | - |
dc.subject | DIABETIC-RATS | - |
dc.subject | ISCHEMIA | - |
dc.subject | PYRUVATE | - |
dc.title | Identification of proteins in hyperglycemia and stroke animal models | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Koh, Phil-Ok | - |
dc.identifier.doi | 10.1016/j.jss.2015.07.020 | - |
dc.identifier.scopusid | 2-s2.0-84938718307 | - |
dc.identifier.wosid | 000366840700049 | - |
dc.identifier.bibliographicCitation | JOURNAL OF SURGICAL RESEARCH, v.200, no.1, pp.365 - 373 | - |
dc.relation.isPartOf | JOURNAL OF SURGICAL RESEARCH | - |
dc.citation.title | JOURNAL OF SURGICAL RESEARCH | - |
dc.citation.volume | 200 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 365 | - |
dc.citation.endPage | 373 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Surgery | - |
dc.relation.journalWebOfScienceCategory | Surgery | - |
dc.subject.keywordPlus | S-ADENOSYLHOMOCYSTEINE HYDROLASE | - |
dc.subject.keywordPlus | CEREBRAL-ARTERY OCCLUSION | - |
dc.subject.keywordPlus | ISOCITRATE DEHYDROGENASE | - |
dc.subject.keywordPlus | GLUCOSE-6-PHOSPHATE ISOMERASE | - |
dc.subject.keywordPlus | GLUCOSE DEPRIVATION | - |
dc.subject.keywordPlus | OXIDATIVE STRESS | - |
dc.subject.keywordPlus | INFARCT VOLUME | - |
dc.subject.keywordPlus | DIABETIC-RATS | - |
dc.subject.keywordPlus | ISCHEMIA | - |
dc.subject.keywordPlus | PYRUVATE | - |
dc.subject.keywordAuthor | Hyperglycemia | - |
dc.subject.keywordAuthor | Stroke | - |
dc.subject.keywordAuthor | Proteomics | - |
dc.subject.keywordAuthor | Streptozotocin | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
Gyeongsang National University Central Library, 501, Jinju-daero, Jinju-si, Gyeongsangnam-do, 52828, Republic of Korea+82-55-772-0533
COPYRIGHT 2022 GYEONGSANG NATIONAL UNIVERSITY LIBRARY. ALL RIGHTS RESERVED.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.