Immunization of Mice with Recombinant Brucella abortus Organic Hydroperoxide Resistance (Ohr) Protein Protects Against a Virulent Brucella abortus 544 Infection
DC Field | Value | Language |
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dc.contributor.author | Hop, Huynh Tan | - |
dc.contributor.author | Reyes, Alisha Wehdnesday Bernardo | - |
dc.contributor.author | Simborio, Hannah Leah Tadeja | - |
dc.contributor.author | Arayan, Lauren Togonon | - |
dc.contributor.author | Min, Won Gi | - |
dc.contributor.author | Lee, Hu Jang | - |
dc.contributor.author | Lee, Jin Ju | - |
dc.contributor.author | Chang, Hong Hee | - |
dc.contributor.author | Kim, Suk | - |
dc.date.accessioned | 2022-12-26T20:31:13Z | - |
dc.date.available | 2022-12-26T20:31:13Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.issn | 1017-7825 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/15758 | - |
dc.description.abstract | In this study, the Brucella abortus ohr gene coding for an organic hydroperoxide resistance protein (Ohr) was cloned into a maltose fusion protein expression system (pMAL), inserted into Escherichia coli, and purified, and its immunogenicity was evaluated by western blot analysis using Brucella-positive mouse sera. The purified recombinant Ohr (rOhr) was treated with adjuvant and injected intraperitoneally into BALB/c mice. A protective immune response analysis revealed that rOhr induced a significant increase in both the IgG1 and IgG2a titers, and IgG2a reached a higher level than IgG1 after the second and third immunizations. Additionally, immunization with rOhr induced high production of IFN-gamma as well as pro-inflammatory cytokines such as TNF, MCP-1, IL-12p70, and IL-6, but a lesser amount of IL-10, suggesting that rOhr predominantly elicited a cell-mediated immune response. In addition, immunization with rOhr caused a significantly higher degree of protection against a virulent B. abortus infection compared with a positive control group consisting of mice immunized with maltose-binding protein. These findings showed that B. abortus rOhr was able to induce both humoral and cell-mediated immunity in mice, which suggested that this recombinant protein could be a potential vaccine candidate for animal brucellosis. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY | - |
dc.subject | DNA VACCINE | - |
dc.subject | L7/L12 | - |
dc.subject | OMP16 | - |
dc.subject | CELLS | - |
dc.title | Immunization of Mice with Recombinant Brucella abortus Organic Hydroperoxide Resistance (Ohr) Protein Protects Against a Virulent Brucella abortus 544 Infection | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Min, Won Gi | - |
dc.contributor.affiliatedAuthor | Lee, Hu Jang | - |
dc.contributor.affiliatedAuthor | Chang, Hong Hee | - |
dc.contributor.affiliatedAuthor | Kim, Suk | - |
dc.identifier.doi | 10.4014/jmb.1505.05028 | - |
dc.identifier.scopusid | 2-s2.0-84955566662 | - |
dc.identifier.wosid | 000369512300023 | - |
dc.identifier.bibliographicCitation | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.26, no.1, pp.190 - 196 | - |
dc.relation.isPartOf | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.title | JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY | - |
dc.citation.volume | 26 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 190 | - |
dc.citation.endPage | 196 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.identifier.kciid | ART002075972 | - |
dc.description.journalClass | 1 | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.description.journalRegisteredClass | kci | - |
dc.relation.journalResearchArea | Biotechnology & Applied Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Biotechnology & Applied Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | DNA VACCINE | - |
dc.subject.keywordPlus | L7/L12 | - |
dc.subject.keywordPlus | OMP16 | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordAuthor | Brucella abortus | - |
dc.subject.keywordAuthor | organic hydroperoxide resistance protein (Ohr) | - |
dc.subject.keywordAuthor | immunization | - |
dc.subject.keywordAuthor | vaccine | - |
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