Detailed Information

Cited 16 time in webofscience Cited 0 time in scopus
Metadata Downloads

Gene expression profiles of putative biomarker candidates in Mycobacterium avium subsp paratuberculosis-infected cattleopen access

Authors
Park, Hyun-EuiShin, Min-KyoungPark, Hong-TaeJung, MyunghwanCho, Yong IlYoo, Han Sang
Issue Date
Jun-2016
Publisher
OXFORD UNIV PRESS
Keywords
Paratuberculosis; Biomarkers; Cattle; Subclinical stage
Citation
PATHOGENS AND DISEASE, v.74, no.4
Indexed
SCI
SCIE
SCOPUS
Journal Title
PATHOGENS AND DISEASE
Volume
74
Number
4
URI
https://scholarworks.gnu.ac.kr/handle/sw.gnu/15440
DOI
10.1093/femspd/ftw022
ISSN
2049-632X
Abstract
This study was conducted to analyze the gene expression of prognostic potential biomarker candidates using the whole blood of cattle naturally infected with Mycobacterium avium subsp. paratuberculosis (MAP). We conducted real-time PCR to evaluate 23 potential biomarker candidates. Experimental animals were divided into four groups based on fecal MAP PCR and serum ELISA. Seven (KLRB1, HGF, MPO, LTF, SERPINE1, S100A8 and S100A9) genes were up-regulated in fecal MAP-positive cattle and three (KLRB1, MPO and S100A9) were up-regulated in MAP-seropositive cattle relative to uninfected cattle. In subclinically infected animals, 17 genes (TFRC, S100A8, S100A9, MPO, GBP6, LTF, KLRB1, SERPINE1, PIGR, IL-10, CXCR3, CD14, MMP9, ELANE, CHI3L1, HP and HGF) were up-regulated compared with the control group. Moreover, six genes (CXCR3, HP, HGF, LTF, TFRC and GBP6) showed significant differences between experimental groups. Taken together, our data suggest that six genes (LTF, HGF, HP, CXCR3, GBP6 and TFRC) played essential roles in the immune response to MAP during the subclinical stage and therefore might be useful as prognostic biomarkers.
Files in This Item
There are no files associated with this item.
Appears in
Collections
College of Medicine > Department of Medicine > Journal Articles

qrcode

Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.

Related Researcher

Researcher Jung, Myung Hwan photo

Jung, Myung Hwan
의과대학 (의학과)
Read more

Altmetrics

Total Views & Downloads

BROWSE