Dermabacter jinjuensis sp nov., a novel species of the genus Dermabacter isolated from a clinical specimen

Abstract

A Gram-stain-positive, catalase-positive, facultatively anaerobic, non-motile, coryneform bacterium, designated strain 32(T), was isolated from a closed pus sample from a patient having finger necrosis in Korea. Strain 32T was considered as representing a novel species according to its initial identification by matrix-assisted laser desorption/ionization-time-of-flight MS. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 321 belonged to the genus Dermabacter and was closely related to Dermabacter hominis DSM 7083(T) (=ATCC 49369(T)) (98.34% similarity). Optimal growth was observed at 30-40 degrees C and pH 7. Growth occurred in the presence of 0-6% (w/v) NaCl. Menaquinones MK-8, MK-7 and MK-9 were the major respiratory quinones. The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, glycolipid and two unknown lipids. The major cellular fatty acids were anteiso-C-17:0, anteiso-C-15:0, iso-C-16:0, and iso-C-15:0. The DNA G+C content of strain 32(T) was 62.58 mol%, and the mean level of DNA-DNA relatedness between strain 32(T) and D. hominis ATCC 49369(T) was 49 +/- 1.6%. Based on the phenotypic and genotypic characteristics, strain 32(T) is confirmed to represent a novel species of the genus Dermabacter, for which the name Dermabacter jinjuensis sp. nov. is proposed. The type strain is 32(T) (=NCCP 16133(T)=DSM 101003(T)).