Effects of cudrania tricuspidata leaf extract on gluten sensitivity

Abstract

In this study, we used Cudrania tricuspidata leaf ethanol extract and confirmed its anti-inflammatory, anti-microbial activities, antioxidant, anti-hyperlipidemia, anti-hypertensive, and anti-cytotoxic effects. Anti-allergic effect on food allergies, which has increased in incidence due to recent westernized eating habits, was confirmed by mouse model-induced gluten sensitivity. For 1,1-diphenyl-2-picrylhydrazyl scavenging activity, C. tricuspidata leaf ethanol extract showed concentration-dependent radical scavenging activity and greater than 80% radical scavenging activity (above 4 mg/mL). In a mouse experiment in which gluten sensitivity was induced by providing gluten diet, the effect of inhibiting weight loss was confirmed in the experimental group administered C. tricuspidata ethanol extract compared to the gluten administration group. In addition, IL-4, an allergic cytokine, was measured in the small intestine and serum using ELISA (enzyme-linked immunosorbent assay), and the results indicate that IL-4 production was significantly reduced in the C. tricuspidata administration group compared to the 200 mg/kg of gluten group. In the lipopolysaccharide-induced RAW 264.7 cell experiment, NO production decreased with increasing concentration of C. tricuspidata leaf ethanol extract. As a result, it was confirmed that C. tricuspidata leaf ethanol extract has high antioxidant ability and mitigates gluten sensitivity-induced inflammation-related allergic reaction. ? 2018, Korean Society of Food Science and Nutrition. All rights reserved.