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제초제 내성 유전자 변형 옥수수 중 PAT단백질에 특이한단크론성 항체의 생산과 특성 확인Production and Characterization of Monoclonal Antibodies Specific to PAT Protein Expressed in Genetically Modified Herbicide-Resistance Maize
- Other Titles
- Production and Characterization of Monoclonal Antibodies Specific to PAT Protein Expressed in Genetically Modified Herbicide-Resistance Maize
- Issue Date
- 2018
- Publisher
- 한국식품위생안전성학회
- Keywords
- GM maize; PAT protein; Hybridoma cell; Monoclonal antibody
- Citation
- 한국식품위생안전성학회지, v.33, no.3, pp 193 - 199
- Pages
- 7
- Indexed
- KCI
- Journal Title
- 한국식품위생안전성학회지
- Volume
- 33
- Number
- 3
- Start Page
- 193
- End Page
- 199
- ISSN
- 1229-1153
2465-9223
- Abstract
- In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.
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Collections - 농업생명과학대학 > 식품공학부 > Journal Articles
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