Heat-stress-modulated induction of NF-kappa B leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model
DC Field | Value | Language |
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dc.contributor.author | Huynh Tan Hop | - |
dc.contributor.author | Arayan, Lauren Togonon | - |
dc.contributor.author | Reyes, Alisha Wehdnesday Bernardo | - |
dc.contributor.author | Tran Xuan Ngoc Huy | - |
dc.contributor.author | Min, Won Gi | - |
dc.contributor.author | Lee, Hu Jang | - |
dc.contributor.author | Rhee, Man Hee | - |
dc.contributor.author | Chang, Hong Hee | - |
dc.contributor.author | Kim, Suk | - |
dc.date.accessioned | 2022-12-26T17:02:07Z | - |
dc.date.available | 2022-12-26T17:02:07Z | - |
dc.date.created | 2022-12-13 | - |
dc.date.issued | 2018-05-25 | - |
dc.identifier.issn | 1471-2180 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gnu/handle/sw.gnu/11638 | - |
dc.description.abstract | Background: Brucella causes a chronic and debilitating infection that leads to great economic losses and a public health burden. In this study, we demonstrated the brucellacidal effect of heat shock mediated by the induction of pro-inflammatory cytokines, reactive oxygen species (ROS) accumulation and apoptosis in murine macrophages and in mice. Results: RAW264.7 cells were incubated at 43 degrees C, and BALB/c mice were subjected to whole body hyperthermia. The data showed a reduction in bacterial survival in the mice after daily heat exposure. This was accompanied by increased levels of cytokines TNF, IL-6, IL-1 beta and IFN-gamma in the sera of themice. Gene expression of NF-kappa B and inducible nitric oxide production were also induced in the mouse splenic cells. In parallel with the bacterial reduction in the mouse model, an increased bactericidal effect was observed in RAW264.7 cells after exposure to heat stress. In addition, the heat stress increased both the nuclear translocation of NF-kappa B and the expression of the heat shock proteins HSP70 and HSP90 in murine macrophages. Furthermore, heat exposure induced the increase of pro-inflammatory cytokines, ROS accumulation and apoptosis but did not affect the production of nitric oxide (NO) in macrophages. Conclusion: This study demonstrated the induction of innate immune responses by heat stress that significantly reduced the intracellular survival of B. abortus in vitro and in vivo. Transcriptional factor NF-kappa B, which is a master regulator, could be termed a key activator of heat-induced immunity against Brucella. The increase in the expression and activation of NF-kappa B in splenic cells and macrophages was followed by enhanced antimicrobial effectors, including cytokines, ROS and NO that may contribute to the reduction of bacterial survival. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | BMC | - |
dc.subject | ACTIVATION | - |
dc.subject | EXPRESSION | - |
dc.subject | LPS | - |
dc.title | Heat-stress-modulated induction of NF-kappa B leads to brucellacidal pro-inflammatory defense against Brucella abortus infection in murine macrophages and in a mouse model | - |
dc.type | Article | - |
dc.contributor.affiliatedAuthor | Min, Won Gi | - |
dc.contributor.affiliatedAuthor | Lee, Hu Jang | - |
dc.contributor.affiliatedAuthor | Chang, Hong Hee | - |
dc.contributor.affiliatedAuthor | Kim, Suk | - |
dc.identifier.doi | 10.1186/s12866-018-1185-9 | - |
dc.identifier.scopusid | 2-s2.0-85047559541 | - |
dc.identifier.wosid | 000433148800002 | - |
dc.identifier.bibliographicCitation | BMC MICROBIOLOGY, v.18 | - |
dc.relation.isPartOf | BMC MICROBIOLOGY | - |
dc.citation.title | BMC MICROBIOLOGY | - |
dc.citation.volume | 18 | - |
dc.type.rims | ART | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.description.isOpenAccess | Y | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.subject.keywordPlus | ACTIVATION | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | LPS | - |
dc.subject.keywordAuthor | B. abortus | - |
dc.subject.keywordAuthor | Heat stress | - |
dc.subject.keywordAuthor | Macrophage | - |
dc.subject.keywordAuthor | NF-kappa B | - |
dc.subject.keywordAuthor | ROS | - |
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