Data on recurrent somatic embryogenesis and in vitro micropropagation of Cnidium officinale Makino

Abstract

Cnidium officinale Makino, a perennial herb of the family Umbelliferae, is a well-known medicinal plant in oriental medicine with antidiabetic, tumor metastatic, antiplatelet, antimicrobial and insecticidal properties. Hence, C officinale does not produce seed the plant tissue culture is the viable alternative for its propagation. Node explant from in vitro grown C officinale Makino was cultured on MS medium supplemented with plant growth regulators (PGRs) like 2,4-Dichlorophenoxyacetic acid (2,4-D) or/and 6-Benzylaminopurine (BA). It was aimed to investigate the optimal concentration and combination of 2,4-D and BA for somatic embryogenesis in node explant of C officinale Makino. The embryogenic callus was induced on node explant after four weeks in MS medium containing 1.5 mg L (1) 2,4-D and 0.5 mg L (1) BA. The translucent white, embryogenic callus was subcultured on the respective medium and individual well-structured somatic embryos were observed. Heart and cotyledon stage embryos were pictured under a stereomicroscope. The individual somatic embryos (SE) were transferred to MS medium without PGRs (MSO) and 100% germination was observed. Repeated subculturing of the embryogenic callus for five months resulted in recurrent somatic embryogenesis but with a gradual decline in number. (C) 2018 The Authors. Published by Elsevier Inc.