Integrated analysis of exoskeletal surface profile and chitin-related gene expression on Macrophthalmus japonicus mud crabs exposed to hexabromocyclododecane

Abstract

Hexabromocyclododecanes (HBCDs), widely used brominated flame retardants, easily accumulate in aquatic organisms such as Macrophthalmus japonicus crabs, which inhabit tidal flat sediments. To analyze the effects of HBCD exposure in chitin-formed exoskeleton, we investigated molecular responses of chitin-related genes as well as physical changes of the exoskeletal surface form as a new biological end-point on M. japonicus. The expression patterns of chitin biosynthesis-, modification-, and degradation-related genes in the gills and hepatopancreases of M. japonicus were also analyzed. Additionally, the survivability and exoskeleton surface profiles of M. japonicus crabs were evaluated. M. japonicus chitin synthase expression was significantly downregulated, whereas that of the chitinase transcript was significantly upregulated upon exposure to all HBCD concentrations on day 7. Contrastingly, the gene expression of chitin deacetylase 1 significantly increased upon exposure to all HBCD concentrations on day 1, and this increase was significantly elevated on day 4. The expression of chitin deacetylase 1 was dose-dependent. Additionally, decreased survival and exoskeleton surface profile changes were observed in M. japonicus crabs exposed to all HBCD concentrations. These results suggest that exposure to HBCD induces changes in the synthesis, modification, and degradation of chitin, a pivotal component of the cuticular exoskeleton, and may disrupt the exoskeletal surface structure in M. japonicus crabs.