https://scholarworks.gnu.ac.kr/handle/sw.gnu/471 Fri, 13 Mar 2026 08:25:28 GMT 2026-03-13T08:25:28Z https://scholarworks.gnu.ac.kr/handle/sw.gnu/82518 Title: Elicitation Strategies for Enhanced Homoplantaginin Production in Salvia plebeia R. Brown In Vitro Cultures and Evaluation of Their Anti-Inflammatory Activities Authors: 박관빈; 김선아; 강주영; 김도현; 차승아; 이지현; 정제나; 강영민; 반영준; 김봉규; 최명석 Abstract: Salvia plebeia R. Brown is a medicinal plant containing various bioactive compounds, including flavonoids, and has been reportedto exhibit diverse pharmacological effects such as anti-inflammatory, antioxidant, and hepatoprotective activities. However, previous studieson S. plebeia have mainly focused on phytochemical identification and pharmacological evaluation, while biotechnological approachesaimed at enhancing the productivity of major bioactive compounds—particularly metabolic regulation and content improvement throughelicitor treatment in in vitro culture systems—remain largely unexplored. In this study, we investigated the effects of elicitor treatmentson the accumulation of homoplantaginin, a major bioactive compound in the leaves of in vitro–cultured S. plebeia, as well as the associatedchanges in anti-inflammatory activity. In vitro–grown plantlets were treated with yeast extract at concentrations of 1, 2, and 5 mg/Land polyethylene glycol (PEG) at concentrations of 1, 2, and 5% (w/v), respectively. Homoplantaginin content was quantitatively analyzedusing high-performance liquid chromatography (HPLC), and anti-inflammatory activity was evaluated by measuring nitric oxide (NO)production inhibition in RAW 264.7 macrophage cells. As a result, the homoplantaginin content was significantly increased in the treatmentwith 2 mg/L yeast extract compared to the control, while the highest NO inhibition activity was observed in the 5% PEG treatment. These findings suggest that elicitor treatment can effectively enhance the production of bioactive compounds and anti-inflammatory activityin in vitro–cultured S. plebeian. Furthermore, this study provides fundamental data supporting the potential industrial application of S. plebeia through further elucidation of metabolic pathways and optimization of culture conditions. Sun, 01 Feb 2026 00:00:00 GMT https://scholarworks.gnu.ac.kr/handle/sw.gnu/82518 2026-02-01T00:00:00Z https://scholarworks.gnu.ac.kr/handle/sw.gnu/81311 Title: Sorption analysis of ethyl formate fumigation for the management of hitchhiking insect pests in miscellaneous goods Authors: Kim, Dongbin; Kwon, Tae-Hyung; Cho, Donghun; Lee, Byung-Ho; Park, Min-Goo Abstract: This study analyses the sorption of ethyl formate on miscellaneous goods to develop a phytosanitary treatment guideline for hitchhiking pests. Moreover, it evaluates whether the concentration of ethyl formate reaches a level that disinfects the target pests. The rising volumes of miscellaneous imported goods, such as sorghum brooms, ground plant materials (such as cocopeat), and processed plant products (including chairs and decorative items), have increased the risk of pest introduction. Laboratory-scale experiments have shown that the sorption rates of EF vary among different product categories, with ground plant materials showing the highest sorption: 65 % on sorghum brooms and 78 % on ground plant materials (P < 0.05). Scale-up fumigation has also shown similar patterns of sorption on different product categories: 76 % on sorghum brooms and 80 % on ground plant materials (P < 0.05). Commercial trials confirmed that EF sorption was higher on ground plant material than on sorghum brooms under actual conditions in imported containers. Although the remaining concentrations on the items differed based on variations in sorption, the concentration-time (Ct) products for all tested items exceeded the LCt(99 %) values (Ct value for disinfecting 99 % of pests) for multiple pest species. Moreover, EF showed a uniform distribution and effective pest control capabilities at a concentration of 70 g/m(3) for 4 h at > 15 degrees C, which reflects the standard treatment period commonly applied for EF fumigation during Korean quarantine processes. These results suggest that EF fumigation is a viable alternative to methyl bromide, thereby offering both environmental benefits and improved worker safety. Thu, 01 Jan 2026 00:00:00 GMT https://scholarworks.gnu.ac.kr/handle/sw.gnu/81311 2026-01-01T00:00:00Z https://scholarworks.gnu.ac.kr/handle/sw.gnu/81895 Title: 산초나무(Zanthoxylum schinifolium) 종자의 품질 평가를 위한 지표성분 Hesperidin 분석: HPLC–MS/MS 기반 연구 Authors: 박지성; 노혜령; 강주영; 김봉규 Abstract: 산초나무(Zanthoxylum schinifolium) 종자는 동아시아에서 향신료 및 약용 자원으로 널리 활용되고 있으나, 화학적으로 규정된 지표물질을 기반으로 한 체계적인 품질평가 기준은 아직 확립되지 않았다. 본 연구에서는 HPLC–DAD와 LC–MS/MS 분석을 통합적으로 적용하여 산초나무 종자의 품질평가를 위한 지표물질로서 hesperidin을 확인하고 검증하였다. 산초 종자의 메탄올 조추출물에 대해 크로마토그래피 분석을 수행한 결과, 주요 플라보노이드 성분들은 UV 흡수 특성과 음이온 ESI–MS/MS 단편화 패턴을 통해 동정되었다. 특히 m/z 609.6[M–H]–의 주요 전구체 이온은 hexose (162Da)와 rhamnose (146Da)의 순차적 중성손실을 거쳐 각각 m/z 463.4 및 301.3의 단편 이온을 생성하였으며, 이러한 단편화 거동은 표준물질 hesperetin-7-O-rutinoside (hesperidin)의 스펙트럼과 완전히 일치하였다. 이후 정량분석을 위해 확립된 HPLC–DAD 분석법은 우수한 직선성(R2 > 0.999), 높은 민감도(LOD 및 LOQ가 허용 범위 내), 그리고 재현성과 정밀도를 나타내어 분석법의 신뢰성을 확인하였다. 또한 여러 배치의 산초나무 종자 시료에서 hesperidin 함량이 일관되게 검출되어, 본 성분이 품질관리 지표물질로서 적합함을 확인하였다. 종합적으로, HPLC–MS/MS 기반 구조 규명과 정량 분석 결과는 hesperidin이 산초나무 종자의 주요 플라보노이드 배당체이며, 화학적 표준화 및 품질평가를 위한 적절한 지표성분임을 명확하게 보여준다. 본 연구는 산초나무 유래 기능성 소재의 산업적 활용과 표준화, 품질 보증을 위한 분석 기반을 제공하는 데 중요한 의의가 있다. Mon, 01 Dec 2025 00:00:00 GMT https://scholarworks.gnu.ac.kr/handle/sw.gnu/81895 2025-12-01T00:00:00Z https://scholarworks.gnu.ac.kr/handle/sw.gnu/80023 Title: Modeling Current and Future Distribution of Ainsliaea acerifolia in Southeastern Korea Using MaxEnt Approach Authors: 강현철; 강주영; 노혜령; 김봉규 Abstract: Ainsliaea acerifolia, a perennial herbaceous plant endemic to forested regions of the Korean Peninsula, has drawn attention for its bioactive properties and potential as a functional food and medicinal resource. Despite its ecological and economic value, little is known about its spatial distribution or habitat preferences under changing climatic conditions. This study aims to quantify the current and future habitat suitability of A. acerifolia in southeastern Korea—including Gyeongsangnam-do, Busan, and Ulsan—using the MaxEnt (Maximum Entropy) species distribution model. A total of 217 presence records were compiled from both GBIF data and field surveys, and seven key environmental variables were selected through multicollinearity screening. Model performance was robust (mean AUC = 0.845), and key predictors included topographic roughness (TRI), precipitation of the driest month (Bio14), and mean diurnal range (Bio2). Under current climate conditions, 31.5% of the study area was identified as suitable habitat (≥0.222), with only 4.7% categorized as core habitat (≥0.6). Future projections under SSP1-2.6, SSP2-4.5, and SSP5-8.5 scenarios indicated substantial habitat contraction, particularly in coastal and lowland areas, with refugial zones likely to persist in mid-elevation inland mountains (500–900 m). These findings suggest that A. acerifolia is highly sensitive to climatic variability and emphasizes the urgent need for conservation strategies focused on climate-resilient refugia and ecological corridors. The study provides a spatial framework for species conservation planning under future climate uncertainty. Fri, 01 Aug 2025 00:00:00 GMT https://scholarworks.gnu.ac.kr/handle/sw.gnu/80023 2025-08-01T00:00:00Z